Bioinformatics Analysis of Cellulose Synthase <i>CesA</i> Gene from <i>Miscanthus lutarioriparius</i>

Based on 3 generations transcriptome sequencing technology-PacBio SMRT single-molecule real-time method, supplemented with 2 generation a complete and accurate unigene library was constructed. After comparing closely related species, the&nbsp;cellulose synthase gene ( CesA )&nbsp; 4 ,&nbsp; 7 9 &nbsp;gene sequences of&nbsp; Miscanthus lutarioriparius &nbsp;were spliced named as&nbsp; MlCesA4 MlCesA7&nbsp; and&nbsp; MlCesA9 . Then, Bioinformatics analysis software used to construct phylogenetic trees of proteins, predict analyze the phosphorylation sites post-translational modifications amino acids, conserved domains proteins.The results showed that sequence lengths MlCesA 4, 7, genes were 2980 bp, 3310 3208 respectively, which to&nbsp; Sorghum bicolor &nbsp;and&nbsp; Zea mays ;&nbsp;cellulose synthase&nbsp;of&nbsp; &nbsp;(MlCesA) have 43 sites, MlCesA7 has 48 sites; is an unstable protein 6 transmembrane domains, are outside membrane inside membrane.; stable both 8 5 membrane. The three all hydrophilic secondary structure dominated by &alpha;-helix random coils.